Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection
Identifieur interne : 001302 ( Main/Exploration ); précédent : 001301; suivant : 001303Moesin: A Cell Membrane Protein Linked with Susceptibility to Measles Virus Infection
Auteurs : Lee M. Dunster [Allemagne] ; Jürgen Schneider-Schaulies [Allemagne] ; Sieglinde Lüffler [Allemagne] ; Wolfgang Lankes [Allemagne] ; Reinhard Schwartz-Albiez [Allemagne] ; Friedrich Lottspeich [Allemagne] ; Volker Ter Meulen [Allemagne]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.
Url:
DOI: 10.1006/viro.1994.1029
Affiliations:
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We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Dunster, Lee M" sort="Dunster, Lee M" uniqKey="Dunster L" first="Lee M." last="Dunster">Lee M. Dunster</name></noRegion><name sortKey="Lankes, Wolfgang" sort="Lankes, Wolfgang" uniqKey="Lankes W" first="Wolfgang" last="Lankes">Wolfgang Lankes</name><name sortKey="Lottspeich, Friedrich" sort="Lottspeich, Friedrich" uniqKey="Lottspeich F" first="Friedrich" last="Lottspeich">Friedrich Lottspeich</name><name sortKey="Luffler, Sieglinde" sort="Luffler, Sieglinde" uniqKey="Luffler S" first="Sieglinde" last="Lüffler">Sieglinde Lüffler</name><name sortKey="Meulen, Volker Ter" sort="Meulen, Volker Ter" uniqKey="Meulen V" first="Volker Ter" last="Meulen">Volker Ter Meulen</name><name sortKey="Schneider Schaulies, Jurgen" sort="Schneider Schaulies, Jurgen" uniqKey="Schneider Schaulies J" first="Jürgen" last="Schneider-Schaulies">Jürgen Schneider-Schaulies</name><name sortKey="Schwartz Albiez, Reinhard" sort="Schwartz Albiez, Reinhard" uniqKey="Schwartz Albiez R" first="Reinhard" last="Schwartz-Albiez">Reinhard Schwartz-Albiez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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